Journal: Scientific Reports

Article Title: Modelling ligand depletion for simultaneous affinity and binding site quantification on cells and tissue

doi: 10.1038/s41598-023-37015-1

Figure Lengend Snippet: Representative binding traces for radioligands. Peptide tracer binding to human GIPR-expressing CHO cells ( a,b ), peptide tracer binding to FAP-expressing HEK cells ( c,d ), low molecular weight tracer binding to PSMA-expressing PC3-PIP cells ( f,g ) and anti-PSMA antibody binding to PSMA-expressing PC3-PIP cells ( i,j ). Binding traces are displayed in black and fitted lines in red: fitted with the 1:1 model ( a,c,f,i ) and with the 1:1 depletion corrected model ( b,d,g,j ), respectively. Cell number used per replicate plotted against number of binding sites (nB) estimated by the depletion model for the corresponding model system ( e,h ).

Article Snippet: PC3-PIP xenograft tissue from mice was obtained from preclinics GmbH (Germany) and stored at − 80 °C.

Techniques: Binding Assay, Expressing, Molecular Weight

Journal: Scientific Reports

Article Title: Modelling ligand depletion for simultaneous affinity and binding site quantification on cells and tissue

doi: 10.1038/s41598-023-37015-1

Figure Lengend Snippet: Representative binding trace for the low molecular weight PSMA-tracer to PSMA-expressing PC3-PIP xenograft tissue. Binding traces are displayed in black and fitted lines in red: fitted with the 1:1 model ( a ) and with the 1:1 depletion corrected model ( b ), respectively.

Article Snippet: PC3-PIP xenograft tissue from mice was obtained from preclinics GmbH (Germany) and stored at − 80 °C.

Techniques: Binding Assay, Molecular Weight, Expressing

Journal: Scientific Reports

Article Title: Modelling ligand depletion for simultaneous affinity and binding site quantification on cells and tissue

doi: 10.1038/s41598-023-37015-1

Figure Lengend Snippet: Summary of kinetic parameters (k a , k d , K D ) and receptor quantification (nB) of the 111 In-PSMA-Tracer on PC3-PIP xenograft tissue samples.

Article Snippet: PC3-PIP xenograft tissue from mice was obtained from preclinics GmbH (Germany) and stored at − 80 °C.

Techniques:

Journal: Pharmaceutics

Article Title: The Future of PSMA-Targeted Radionuclide Therapy: An Overview of Recent Preclinical Research

doi: 10.3390/pharmaceutics11110560

Figure Lengend Snippet: Overview of PSMA-targeting small molecule inhibitors that have been conjugated with an albumin binding domain and their in vivo biodistribution data in mice. * Values had to be based on graphs rather than exact data as they were not present in the reference.

Article Snippet: DOTA-EB-MCG , Yttrium-90 , truncated Evans blue , Athymic Nude-Foxn1nu, Envigo + PC3-PIP tumors , 3.7–5.1 MBq , ±4 * , [ ] .

Techniques: Binding Assay, In Vivo, Mouse Assay, Injection